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MicroRNA‐145 suppresses mouse granulosa cell proliferation by targeting activin receptor IB
Author(s) -
Yan Guijun,
Zhang Lianxiao,
Fang Ting,
Zhang Qun,
Wu Shaogen,
Jiang Yue,
Sun Haixiang,
Hu Yali
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.06.048
Subject(s) - microrna , cell growth , three prime untranslated region , messenger rna , untranslated region , biology , microbiology and biotechnology , ectopic expression , folliculogenesis , western blot , gene , genetics , embryo , cryopreservation
MicroRNAs (miRNAs) are a class of 21‐ to 25‐nucleotide non‐coding RNAs, some of which are important gene regulators involved in folliculogenesis. In this study, we used CCK‐8, real‐time PCR and Western blot assays to demonstrate that miR‐145 inhibits mouse granulosa cell (mGC) proliferation. Combined with the results of luciferase reporter assays that studied the 3′‐untranslated region of ACVRIB mRNA, these assays identified ACVRIB as a direct target of miR‐145. The ectopic expression of miR‐145 reduced the levels of both ACVRIB mRNA and protein and also interfered with activin‐induced Smad2 phosphorylation. Altogether, this study revealed that miR‐145 suppresses mGC proliferation by targeting ACVRIB.