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miR395 is a general component of the sulfate assimilation regulatory network in Arabidopsis
Author(s) -
Matthewman Colette A.,
Kawashima Cintia G.,
Húska Dalibor,
Csorba Tibor,
Dalmay Tamas,
Kopriva Stanislav
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.06.044
Subject(s) - assimilation (phonology) , sulfate , cysteine , buthionine sulfoximine , arabidopsis , biochemistry , glutathione , chemistry , nitrogen assimilation , microbiology and biotechnology , biology , gene , enzyme , mutant , philosophy , linguistics , organic chemistry
In plants, microRNAs play an important role in many regulatory circuits, including responses to environmental cues such as nutrient limitations. One such microRNA is miR395, which is strongly up‐regulated by sulfate deficiency and targets two components of the sulfate uptake and assimilation pathway. Here we show that miR395 levels are affected by treatments with metabolites regulating sulfate assimilation. The precursor of cysteine, O ‐acetylserine, which accumulates during sulfate deficiency, causes increase in miR395 accumulation. Feeding plants with cysteine, which inhibits sulfate uptake and assimilation, induces miR395 levels while buthionine sulfoximine, an inhibitor of glutathione synthesis, lowers miR395 expression. Thus, miR395 is an integral part of the regulatory network of sulfate assimilation.