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Involvement of hepatitis B virus X gene (HBx) integration in hepatocarcinogenesis via a recombination of HBx/ Alu core sequence/subtelomeric DNA
Author(s) -
Zhang Xuan,
You Xiaona,
Li Nan,
Zhang Weiying,
Gagos Sarantis,
Wang Qi,
Banos Aggelos,
Cai Na,
Zhang Huakun,
Zhang Hang,
Zhang Xuezhi,
Shan Changliang,
Qiu Liyan,
Zhang Shuai,
Lv Na,
Chen Minshan,
Du Yumei,
Xia Jianchuan,
Ye Lihong,
Zhang Xiaodong
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.06.039
Subject(s) - hbx , subtelomere , hepatitis b virus , biology , alu element , gene , transformation (genetics) , virology , dna , microbiology and biotechnology , telomere , virus , genetics , genome , human genome
The significance of hepatitis B virus (HBV) DNA‐based integration in hepatocarcinogenesis is poorly understood. In the present study, we investigated whether the integration of HBV X gene (HBx) is involved in the event. Our finding showed that the integration of HBx fragment (316–462 bp/262–462 bp) was able to transform human immortalized normal liver LO2 cells using a cell model of HBx‐integration. We identified that the recombination, HBx/ Alu core sequence/subtelomeric DNA, was required for the transformation, which could be detected in 5 out of 44 clinical HBx‐positive hepatocellular carcinoma tissues. Thus, we conclude that HBx integration is involved in the hepatocarcinogenesis.