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Evidence for rapid uptake of d ‐galacturonic acid in the yeast Saccharomyces cerevisiae by a channel‐type transport system
Author(s) -
Souffriau Ben,
den Abt Tom,
Thevelein Johan M.
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.06.012
Subject(s) - saccharomyces cerevisiae , yeast , chemistry , biochemistry , channel (broadcasting) , computer science , computer network
d ‐Galacturonic acid is a major component of pectins but cannot be metabolized by Saccharomyces cerevisiae . It is assumed not to be taken up. We show that yeast displays surprisingly rapid low‐affinity uptake of d ‐galacturonic acid, strongly increasing with decreasing extracellular pH and without saturation up to 1.5 M. There was no intracellular concentration above the extracellular level and transport was reversible. Among more than 160 single and multiple deletion mutants in channels and transporters, no strain was affected in d ‐galacturonic acid uptake. The uptake was not inhibited by any compound tested as candidate competitive inhibitor, including d ‐glucuronic acid, which was also transported. The characteristics of d ‐galacturonic acid uptake are consistent with involvement of a channel‐type system, probably encoded by multiple genes.