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The proline metabolism intermediate Δ 1 ‐pyrroline‐5‐carboxylate directly inhibits the mitochondrial respiration in budding yeast
Author(s) -
Nishimura Akira,
Nasuno Ryo,
Takagi Hiroshi
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.05.056
Subject(s) - mitochondrion , yeast , biochemistry , saccharomyces cerevisiae , respiration , cellular respiration , superoxide , programmed cell death , biology , metabolism , proline , chemistry , botany , enzyme , apoptosis , amino acid
The proline metabolism intermediate Δ 1 ‐pyrroline‐5‐carboxylate (P5C) induces cell death in animals, plants and yeasts. To elucidate how P5C triggers cell death, we analyzed P5C metabolism, mitochondrial respiration and superoxide anion generation in the yeast Saccharomyces cerevisiae . Gene disruption analysis revealed that P5C‐mediated cell death was not due to P5C metabolism. Interestingly, deficiency in mitochondrial respiration suppressed the sensitivity of yeast cells to P5C. In addition, we found that P5C inhibits the mitochondrial respiration and induces a burst of superoxide anions from the mitochondria. We propose that P5C regulates cell death via the inhibition of mitochondrial respiration.