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HSC90 is required for nascent hepatitis C virus core protein stability in yeast cells
Author(s) -
Kubota Naoko,
Inayoshi Yasutaka,
Satoh Naoko,
Fukuda Takashi,
Iwai Kenta,
Tomoda Hiroshi,
Kohara Michinori,
Kataoka Kazuhiro,
Shimamoto Akira,
Furuichi Yasuhiro,
Nomoto Akio,
Naganuma Akira,
Kuge Shusuke
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.05.023
Subject(s) - yeast , organelle , core protein , microbiology and biotechnology , saccharomyces cerevisiae , core (optical fiber) , budding yeast , chemistry , biology , virology , biochemistry , materials science , composite material
Hepatitis C virus core protein (Core) contributes to HCV pathogenicity. Here, we demonstrate that Core impairs growth in budding yeast. We identify HSP90 inhibitors as compounds that reduce intracellular Core protein level and restore yeast growth. Our results suggest that HSC90 (Hsc82) may function in the protection of the nascent Core polypeptide against degradation in yeast and the C‐terminal region of Core corresponding to the organelle‐interaction domain was responsible for Hsc82‐dependent stability. The yeast system may be utilized to select compounds that can direct the C‐terminal region to reduce the stability of Core protein.