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Induction of epithelial‐mesenchymal transition with O‐glycosylated oncofetal fibronectin
Author(s) -
Ding Yao,
Gelfenbeyn Kirill,
Freire-de-Lima Leonardo,
Handa Kazuko,
Hakomori Sen-itiroh
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.05.020
Subject(s) - epithelial–mesenchymal transition , fibronectin , oncofetal antigen , transforming growth factor , mesenchymal stem cell , monoclonal antibody , biology , transition (genetics) , chemistry , microbiology and biotechnology , cancer research , antibody , immunology , extracellular matrix , biochemistry , gene , tumor associated antigen
Epithelial‐mesenchymal transition (EMT) has been shown to play a key role in embryogenesis and cancer progression. We previously found that fibronectin (FN) carrying O ‐GalNAc at a specific site is selectively expressed in cancer and fetal cells/tissues, and termed oncofetal FN (onfFN). Here, we show that (i) a newly‐established monoclonal antibody against FN lacking the O ‐GalNAc, termed normalFN (norFN), is useful for isolation of onfFN, (ii) onfFN, but not norFN, can induce EMT in human lung carcinoma cells, (iii) onfFN has a synergistic effect with transforming growth factor (TGF)β1 in EMT induction.