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Regulatory mechanism of PAC1 gene expression via Sp1 by nerve growth factor in PC12 cells
Author(s) -
Miura Ayako,
Odahara Naoko,
Tominaga Aiko,
Inoue Kazuhiko,
Kambe Yuki,
Kurihara Takashi,
Miyata Atsuro
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.05.009
Subject(s) - adenylate kinase , promoter activity , promoter , nerve growth factor , luciferase , reporter gene , chemistry , gene expression , mapk/erk pathway , gene , microbiology and biotechnology , regulation of gene expression , sp1 transcription factor , biology , signal transduction , enzyme , transfection , biochemistry , receptor
In addition to VPAC1 and VPAC2, PAC1 is involved in the pleiotropic action of pituitary adenylate cyclase activating polypeptide (PACAP) in the CNS. A luciferase reporter assay for the human PAC1 gene (−2160/+268) revealed that NGF treatment significantly augments the promoter activity of the PAC1 gene. Moreover, the Sp1 site at −282/−273 was shown to be essential for the NGF‐augmented promoter activity of the PAC1 gene. Treatment with U0126, an MEK inhibitor, or Mithramycin A, an Sp1 inhibitor, significantly attenuated promoter activity. These results indicate that activation of Sp1 by the Ras/MAPK pathway might participate in neuron specific expression of the PAC1 gene.