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Live imaging of active fluorophore labelled Wnt proteins
Author(s) -
Holzer Tatjana,
Liffers Katrin,
Rahm Karolin,
Trageser Benjamin,
Özbek Suat,
Gradl Dietmar
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.04.035
Subject(s) - wnt signaling pathway , fluorophore , secretion , green fluorescent protein , fusion protein , microbiology and biotechnology , extracellular , proteases , chemistry , live cell imaging , biology , biochemistry , fluorescence , cell , enzyme , signal transduction , gene , recombinant dna , physics , quantum mechanics
Here, we have generated a Wnt2b–EGFP fusion protein that retains functionality in bona fide Wnt activity assays, although the secreted protein is rapidly cleaved by extracellular proteases. We can show with this new tool that Wnt2b–EGFP moves along the microtubules of Wnt producing cells and that this directed movement is essential for the secretion of active Wnt protein.
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