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Calmodulin regulates the translocation of Grb7 into the nucleus
Author(s) -
García-Palmero Irene,
Villalobo Antonio
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.04.017
Subject(s) - calmodulin , nucleus , nuclear localization sequence , chromosomal translocation , nls , microbiology and biotechnology , cell nucleus , chemistry , signal transduction , biophysics , biology , biochemistry , gene , enzyme
We describe in this report the presence of a nuclear localization signal (NLS) overlapping the calmodulin‐binding domain (CaM‐BD) of the growth factor receptor bound protein 7 (Grb7). We show that deletion of the CaM‐BD of Grb7 prevents its nuclear localization, and that its Src homology 2 (SH2) domain might participate as well in the translocation process. Also, treating cells with the CaM antagonist N ‐(6‐aminohexyl)‐5‐chloro‐1‐naphthalenesulfonamide (W‐7) enhances the presence of Grb7 in the nucleus. We propose that CaM inhibits the translocation of Grb7 to the nucleus after binding to its CaM‐BD and therefore occluding its overlapping NLS.