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Expression of BMP‐2 and Ets1 in BMP‐2‐stimulated mouse pre‐osteoblast differentiation is regulated by microRNA‐370
Author(s) -
Itoh Tomohiro,
Ando Masashi,
Tsukamasa Yasuyuki,
Akao Yukihiro
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.04.014
Subject(s) - osteoblast , bone morphogenetic protein 2 , microbiology and biotechnology , microrna , cellular differentiation , bone morphogenetic protein , biology , chemistry , gene , genetics , in vitro
MicroRNAs (miRs) regulate several biological functions such as cell growth, cell differentiation, and carcinogenesis, by binding to the 3′‐untranslated regions (3′‐UTR) of specific target genes, in order to repress translation or promote degradation of the transcribed mRNAs. In the present study, using microRNA array and in silico analyses, we found that miR‐370 regulates the expression of bone morphogenetic protein‐2 (BMP‐2) and V‐ets Erythroblastosis Virus E26 Oncogene Homolog 1 (Ets1) in BMP‐2‐stimulated murine pre‐osteoblast MC3T3‐E1 cell differentiation. The enforced expression of mature miR‐370 in MC3T3‐E1 cells or primary osteoblast cells remarkably attenuated BMP‐2‐induced pre‐osteoblast differentiation. To ascertain the mechanisms underlying the regulation of osteoblast differentiation by miR‐370, we hypothesized a BMP‐2‐Ets1‐PTHrP feed‐forward loop regulatory mechanism.