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Constitutively active Cullin‐RING‐Ligases fail to rescue loss of NEDD8 conjugation in Schizosaccharomyces pombe
Author(s) -
Girdwood David,
Robertson Morag,
Gordon Colin
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.04.011
Subject(s) - cullin , neddylation , nedd8 , schizosaccharomyces pombe , schizosaccharomyces , microbiology and biotechnology , ubiquitin ligase , dna ligase , ubiquitin , biology , chemistry , biochemistry , yeast , saccharomyces cerevisiae , enzyme , gene
In fission yeast, the only known essential function of Ned8p is the modification of the cullin, Pcu1p, and subsequent Cullin‐RING‐Ligase (CRL) activation and substrate ubiquitination. We show here that a functional Pcu1p mutant, deleted for its C‐terminal autoinhibitory domain, which negates the requirement of neddylation for ligase activity, is unable to rescue the loss of neddylation. These findings suggest that the neddylation of non‐cullin substrate(s) are required for Schizosaccharomyces pombe viability.