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Nemo‐like kinase promotes etoposide‐induced apoptosis of male germ cell‐derived GC‐1 cells in vitro
Author(s) -
Cheng Xiaowen,
Liang Junbo,
Teng Yu,
Fu Jun,
Miao Shiying,
Zong Shudong,
Wang Linfang
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.04.005
Subject(s) - germ cell , gene knockdown , apoptosis , etoposide , microbiology and biotechnology , kinase , biology , rna interference , cancer research , spermatogenesis , gene , rna , genetics , endocrinology , chemotherapy
Spermatogenesis is an extremely intricate process that is tightly regulated and orchestrated by a series of well‐coordinated gene expression programmes. Nemo‐like kinase (NLK) is an evolutionarily conserved serine/threonine kinase that functions in a wide variety of developmental events. Nevertheless, the function of NLK in spermatogenesis has not been investigated. In this study, we found that the distribution of NLK in mice exhibited a dynamic change during testicular development and gradually became concentrated in the acrosomes of elongated spermatids. NLK overexpression promoted etoposide‐induced apoptosis of male germ cell‐derived GC‐1 cells, while knockdown of NLK by RNA interference (RNAi) attenuated etoposide‐induced apoptosis. Our findings suggest that NLK plays an important role in etoposide‐induced germ cell apoptosis and may be associated with spermatogenesis.