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Crystal structure of Pyrococcus furiosus PF2050, a member of the DUF2666 protein family
Author(s) -
Han Byeong-Gu,
Jeong Kyung-Chae,
Cho Jea-Won,
Jeong Byung-Cheon,
Song Hyun Kyu,
Lee Jae Young,
Shin Dong Hyuk,
Lee Sangho,
Lee Byung Il
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.04.004
Subject(s) - pyrococcus furiosus , antiparallel (mathematics) , crystallography , crystal structure , chemistry , protein structure , biophysics , biology , biochemistry , archaea , gene , physics , quantum mechanics , magnetic field
Pyrococcus furiosus PF2050 is an uncharacterized putative protein that contains two DUF2666 domains. Functional and structural studies of PF2050 have not previously been performed. In this study, we determined the crystal structure of PF2050. The structure of PF2050 showed that the two DUF2666 domains interact tightly, forming a globular structure. Each DUF2666 domain comprises an antiparallel β‐sheet and an α‐helical bundle. One side of the PF2050 structure has a positively charged basic cleft, which may have a DNA‐binding function. Furthermore, we confirmed that PF2050 interacts with circular and linear dsDNA.