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Esre : A novel essential non‐coding RNA in Escherichia coli
Author(s) -
Chen Zhichao,
Wang Yi,
Li Yarong,
Li Yue,
Fu Nan,
Ye Jiang,
Zhang Huizhan
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.03.010
Subject(s) - escherichia coli , gene , transfer rna , genetics , rna , biology , plasmid , computational biology
YigP gene (GeneID: 948915) locates between ubiquinone biosynthetic genes ubiE and ubiB in Escherichia coli . GeneBank annotates yigP as a putative protein‐coding gene. In this study, we found a new essential sRNA gene, esre , locates within the region of yigP . The E. coli strain with inactive esre must rely on a complementary plasmid to survive. Moreover, RACE experiments showed esre encodes an RNA molecule of 252 nt. Further experiments revealed esre gene is immune to frame shift mutations and the function of esre depends mostly on the RNA secondary structure, which are typical traits of sRNA. Since it is difficult to predict the target of an essential sRNA, more research is needed to reveal the function and mechanism of esre .