Premium
Involvement of microRNA‐93, a new regulator of PTEN/Akt signaling pathway, in regulation of chemotherapeutic drug cisplatin chemosensitivity in ovarian cancer cells
Author(s) -
Fu Xin,
Tian Jing,
Zhang Lei,
Chen Ying,
Hao Quan
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.03.006
Subject(s) - pten , cisplatin , ovarian cancer , microrna , cancer research , carcinogenesis , protein kinase b , regulator , biology , cell , cancer , cell growth , pi3k/akt/mtor pathway , signal transduction , microbiology and biotechnology , chemotherapy , gene , genetics
The mechanisms underlying ovarian cancer cell resistance to cisplatin (CDDP) are not fully understood. MicroRNAs (miRNAs) play important roles in tumorigenesis and drug resistance. In this paper, we utilized microRNA array and real‐time PCR to show that miR‐93 is significantly up‐regulated in cisplatin‐resistant ovarian cancer cells. In vitro assays show that over‐expression and knock‐down of miR‐93 regulate apoptotic activity, and thereby cisplatin chemosensitivity, in ovarian cells. Furthermore, we found that miR‐93 can directly target PTEN, and participates in the regulation of the AKT signaling pathway. MiR‐93 inversely correlates with PTEN expression in CDDP‐resistant and sensitive human ovarian cancer tissues. These results may have implications for therapeutic strategies aiming to overcome ovarian cancer cell resistance to cisplatin.