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The inhibitory effect of miRNA‐1 on ET‐1 gene expression
Author(s) -
Li Dong,
He Bin,
Zhang Huan,
Shan Shi-Fu,
Liang Qing,
Yuan Wen-Jun,
Ren An-Jing
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.02.044
Subject(s) - microrna , gene expression , gene , reporter gene , biology , regulation of gene expression , luciferase , microbiology and biotechnology , three prime untranslated region , untranslated region , genetics , transfection , messenger rna
There is a close correlation between endothelin‐1 (ET‐1) and microRNA‐1 (miRNA‐1) expression in the heart, but whether ET‐1 expression is regulated by miRNA‐1 warrants further research. Our results revealed multiple clues suggesting that miRNA‐1 may participate in inhibiting ET‐1 gene expression. The inhibitory effect of miRNA‐1 on recombinant luciferase reporter gene was mediated by the target sequence at the 127th nucleotide on ET‐1 3′UTR. We further confirmed that miRNA‐1 could inhibit endogenous ET‐1 gene expression at the post‐transcriptional level. Our study provides a new perspective on the regulatory mechanism of ET‐1 gene.