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Krüppel‐like factor 4 inhibits the expression of interleukin‐1 beta in lipopolysaccharide‐induced RAW264.7 macrophages
Author(s) -
Liu Junwen,
Yang Tao,
Liu Ying,
Zhang Huali,
Wang Kangkai,
Liu Meidong,
Chen Guangwen,
Xiao Xianzhong
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.02.003
Subject(s) - klf4 , microbiology and biotechnology , peripheral blood mononuclear cell , lipopolysaccharide , transfection , transcription factor , interleukin , transcription (linguistics) , chemistry , biology , immunology , cytokine , gene , biochemistry , in vitro , sox2 , linguistics , philosophy
RAW264.7 macrophages and human peripheral blood mononuclear cells were treated with LPS to determine the expression of KLF4 and release of IL‐1β. A full‐length cDNA or short interference RNA of KLF4 was transfected into RAW264.7 macrophages; the expression and release of IL‐1β were analyzed. The transcription and DNA binding activities of KLF4 to the IL‐1β promoter were detected further. The results showed LPS treatment resulted in the increase of KLF4 level and IL‐1β release; KLF4 overexpression decreased the expression of IL‐1β, while KLF4 inhibition increased the expression of IL‐1β; overexpression of KLF4 promoted the DNA binding activity of KLF4 to the IL‐1β promoter and attenuated the transcription of IL‐1β promoter, indicating an important role of KLF4 in regulating expression of IL‐1β.