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Requirement for Pak3 in Rac1‐induced organization of actin and myosin during Drosophila larval wound healing
Author(s) -
Baek Seung Hee,
Cho Hae Weon,
Kwon Young-Chang,
Lee Ji Hyun,
Kim Moon Jong,
Lee Hyangkyu,
Choe Kwang-Min
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.01.061
Subject(s) - rac1 , myosin , microbiology and biotechnology , myosin light chain kinase , wound healing , actin , biology , pak1 , rho associated protein kinase , chemistry , signal transduction , genetics
Rho‐family small GTPases regulate epithelial cell sheet migration by organizing actin and myosin during wound healing. Here, we report that Pak3, but not Pak1, is a downstream target protein for Rac1 in wound closure of the Drosophila larval epidermis. Pak3‐deficient larvae failed to close a wound hole and this defect was not rescued by Pak1 expression, indicating differential functions of the two proteins. Pak3 localized to the wound margin, which selectively required Rac1. Pak3‐deficient larvae showed severe defects in actin‐myosin organization at the wound margin and in submarginal cells, which was reminiscent of the phenotypes of Rac1‐deficient larvae. These results suggest that Pak3 specifically mediates Rac1 signaling in organizing actin and myosin during Drosophila epidermal wound healing.