Premium
Imprinted DNA methylation reprogramming during early mouse embryogenesis at the Gpr1 ‐ Zdbf2 locus is linked to long cis ‐intergenic transcription
Author(s) -
Kobayashi Hisato,
Sakurai Takayuki,
Sato Shun,
Nakabayashi Kazuhiko,
Hata Kenichiro,
Kono Tomohiro
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.01.059
Subject(s) - reprogramming , dna methylation , genomic imprinting , biology , intergenic region , transcription (linguistics) , genetics , microbiology and biotechnology , genome , gene , gene expression , linguistics , philosophy
The paternally‐expressed imprinted genes Gpr1 and Zdbf2 form a gene cluster wherein the imprinted‐methylated regions of these two genes differ. We identified a novel, paternally expressed, long intergenic non‐coding Zdbf2 variant ( Zdbf2linc ) transcribed from maternally methylated Gpr1 DMR during early embryogenesis in the mouse. While the Gpr1 DMR displayed biallelic hypermethylation, Zdbf2linc expression was rarely observed in the post‐gastrulation, despite a positive correlation between the methylation of Zdbf2 DMRs and the mono‐allelic transcription of the original Zdbf2 coding variant. Furthermore, lack of the maternal methylation imprint resulted in the biallelic expression of both coding and non‐coding Zdbf2 transcripts as well as complete methylation of Zdbf2 DMRs. Globally, our findings suggest the role of Zdbf2linc in the establishment of secondary epigenetic modifications after implantation.