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Construction of synthetic regulatory networks in yeast
Author(s) -
Blount Benjamin A.,
Weenink Tim,
Ellis Tom
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.01.053
Subject(s) - saccharomyces cerevisiae , yeast , synthetic biology , escherichia coli , computational biology , simple (philosophy) , biology , biochemical engineering , computer science , genetics , gene , engineering , philosophy , epistemology
Yeast species such as Saccharomyces cerevisiae have been exploited by humans for millennia and so it is therefore unsurprising that they are attractive cells to re‐engineer for industrial use. Despite many beneficial traits yeast has for synthetic biology, it currently lags behind Escherichia coli in the number of synthetic networks that have been described. While the eukaryotic nature of yeast means that its regulation is not as simple to predict as it is for E. coli , once initial considerations have been made yeast is pleasingly tractable. In this review we provide a loose guide for constructing and implementing synthetic regulatory networks in S. cerevisiae using examples from previous research to highlight available resources, specific considerations and potential future advances.