Premium
Insights into differential modulation of receptor function by hinge region using novel agonistic lutropin receptor and inverse agonistic thyrotropin receptor antibodies
Author(s) -
Majumdar Ritankar,
Railkar Reema,
Dighe Rajan R
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.01.052
Subject(s) - receptor , thyrotropin receptor , epitope , hinge , inverse agonist , agonist , chemistry , agonistic behaviour , mutant , antibody , endocrinology , medicine , microbiology and biotechnology , biology , autoantibody , immunology , biochemistry , physics , gene , classical mechanics , psychiatry , aggression
We report two antibodies, scFv 13B1 and MAb PD1.37, against the hinge regions of LHR and TSHR, respectively, which have similar epitopes but different effects on receptor function. While neither of them affected hormone binding, with marginal effects on hormone response, scFv 13B1 stimulated LHR in a dose‐dependent manner, whereas MAb PD1.37 acted as an inverse agonist of TSHR. Moreover, PD1.37 could decrease the basal activity of hinge region CAMs, but had varied effects on those present in ECLs, whereas 13B1 was refractory to any CAMs in LHR. Using truncation mutants and peptide phage display, we compared the differential roles of the hinge region cysteine box‐2/3 as well as the exoloops in the activation of these two homologus receptors.