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Use of Kikume green–red fusions to study the influence of pharmacological chaperones on trafficking of G protein‐coupled receptors
Author(s) -
Ridelis Ingrid,
Schmidt Antje,
Teichmann Anke,
Furkert Jens,
Wiesner Burkhard,
Schülein Ralf
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.01.043
Subject(s) - receptor , microbiology and biotechnology , g protein coupled receptor , population , chemistry , mutant , protein folding , biology , biochemistry , gene , medicine , environmental health
In this study we demonstrate that the photoconvertible monomeric Kikume green–red (mKikGR) protein is suitable to study trafficking of G protein‐coupled receptors. Taking mKikGR‐tagged mutants of the vasopressin V 2 receptor (V 2 R) as models, we analyzed whether the V 2 R‐specific pharmacological chaperone SR121463B influences receptor folding on a co‐ or post‐translational level. Misfolded mKikGR‐tagged V 2 Rs were completely photoconverted in the early secretory pathway yielding a red receptor population (already synthesized receptors) and an arising green receptor population (newly synthesized receptors). Trafficking of both receptor populations could be rescued by treatment with SR121463B demonstrating that the substance can act co‐ and post‐translationally.