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Overexpression of lalA , a paralog of labA , is capable of affecting both circadian gene expression and cell growth in the cyanobacterium Synechococcus elongatus PCC 7942
Author(s) -
Taniguchi Yasuhito,
Nishikawa Tomoe,
Kondo Takao,
Oyama Tokitaka
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.01.035
Subject(s) - circadian rhythm , biology , gene , mutant , phenotype , gene expression , circadian clock , microbiology and biotechnology , synechococcus , cell , cell growth , genetics , endocrinology , cyanobacteria , bacteria
In the cyanobacterium Synechococcus elongatus , LabA negatively regulates circadian gene expression under the control of Kai‐protein‐based clock. Here we conducted a molecular genetic analysis of lalA , a paralog of labA . Although a lalA loss of function mutant did not exhibit any apparent phenotype under our experimental conditions, lalA overexpression inhibited cell growth and decreased cell viability. Moderate lalA overexpression brought about abnormalities in circadian gene expression: reduced amplitude of kaiBC expression rhythm, and altered peak and trough timing of psbAI and kaiA expression rhythms. These results imply that lalA is capable of affecting circadian gene expression and cell growth.