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Transcription‐independent role of Bach1 in mitosis through a nuclear exporter Crm1‐dependent mechanism
Author(s) -
Li Jie,
Shiraki Takuma,
Igarashi Kazuhiko
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.01.028
Subject(s) - mitosis , nuclear export signal , biology , transcription (linguistics) , microbiology and biotechnology , chromatin , cytoplasm , transcription factor , repressor , genetics , heterochromatin , cell nucleus , dna , gene , linguistics , philosophy
The transcriptional repressor Bach1 mediates various stress responses. Despite its role in transcription, Bach1 is predominantly exported to the cytoplasm in a Crm1‐dependent manner, but the functional role of its cytoplasmic retention is still unclear. We found that Bach1 was also excluded from mitotic chromatin by a C‐terminal cytoplasmic localization sequence dependent and leptomycin B sensitive process. Bach1 depletion resulted in disordered mitotic chromosome alignment, which was rescued by Bach1 mutants lacking the BTB or DNA binding domains, suggesting its transcription‐independent mechanism. We thus revealed a novel role of Bach1 in the regulation of mitotic chromosome dynamics.