Premium
SNPs of hemocyanin C‐terminal fragment in shrimp Litopenaeus vannamei
Author(s) -
Zhao Xianliang,
Guo Lingling,
Zhang Yueling,
Liu Yao,
Zhang Xiaoyu,
Lun Jingsheng,
Chen Jiehui,
Li Yuanyou
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.12.038
Subject(s) - litopenaeus , shrimp , biology , hemocyanin , genetics , single nucleotide polymorphism , vibrio parahaemolyticus , nucleic acid sequence , gene , bacteria , genotype , ecology , antigen
In this study, we identified a variable region in the C‐terminus of hemocyanin from the shrimp Litopenaeus vannamei (2288–2503 bp, HcSC) by sequence alignments. A total of 13 SNPs were identified by PCR‐SSCP and HcSC clone sequencing. The SSCP patterns of HcSC could be modulated in Vibro parahaemolyticus ‐treated shrimps. A novel SSCP band with four SNP sites was identified in V. parahaemolyticus ‐resistant shrimps. More importantly, three of these four SNPs introduced variations in amino acid sequence and possibly secondary structure of the HcSC polypeptide and resulted in a higher agglutinative activity against seven pathogenic bacteria. These results suggest that the C‐terminus of shrimp L. vannamei hemocyanin possesses SNPs, which may be related to shrimp resistance to different pathogens.