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Double‐stranded RNA induces S100 gene expression by a cycloheximide‐sensitive factor
Author(s) -
Voss Andreas,
Gescher Kirsten,
Hensel Andreas,
Nacken Wolfgang,
Zänker Kurt S.,
Kerkhoff Claus
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.12.022
Subject(s) - s100a9 , cycloheximide , gene expression , biology , rna silencing , gene , rna , s100a8 , brefeldin a , microbiology and biotechnology , interferon , rna interference , cell , immunology , protein biosynthesis , biochemistry , golgi apparatus
Viral double‐stranded RNA (dsRNA) and its synthetic analog polyI:C are recognized via multiple pathways and induce the expression of genes related to inflammation. In the present study, we demonstrated the polyI:C‐induced gene expression of the damage associated molecular pattern (DAMP) molecules S100A8 and S100A9, while other S100 genes were not affected. Cycloheximide and Brefeldin A treatment revealed both the expression of S100A8 and S100A9 as secondary response genes and the involvement of polyI:C‐induced cytokines herein. Several type I and type III interferons such as IFNβ, IL‐20, IL‐24, and IFNλ/IL‐29 were expressed in response to polyI:C, however, they failed to induce S100A8 and S100A9 gene expression. These data indicate the involvement of the danger molecule S100A8/A9 in the resistance against viruses.