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d ‐Lactate dehydrogenase as a marker gene allows positive selection of transgenic plants
Author(s) -
Wienstroer Judith,
Engqvist Martin K.M.,
Kunz Hans-Henning,
Flügge Ulf-Ingo,
Maurino Veronica G.
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.11.020
Subject(s) - lactate dehydrogenase , transgene , arabidopsis thaliana , germination , genetically modified crops , seedling , biology , dehydrogenase , marker gene , gene , selection (genetic algorithm) , biochemistry , enzyme , botany , mutant , artificial intelligence , computer science
d ‐Lactate negatively affects Arabidopsis thaliana seedling development in a concentration‐dependent manner. At media d ‐lactate concentrations greater than 5–10 mM the development of wild‐type plants is arrested shortly after germination whereas plants overexpressing the endogenous d ‐lactate dehydrogenase ( d ‐LDH) detoxify d ‐lactate to pyruvate and survive. When the transgenic plants are further transferred to normal growth conditions they develop indistinguishably from the wild type. Thus, d ‐LDH was successfully established as a new marker in A. thaliana allowing selecting transgenic plants shortly after germination. The selection on d ‐lactate containing media adds a new optional marker system, which is especially useful if the simultaneous selection of multiple constructs is desired.