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Changes in cell migration of mesenchymal cells during osteogenic differentiation
Author(s) -
Ichida Masanori,
Yui Yoshihiro,
Yoshioka Kiyoko,
Tanaka Takaaki,
Wakamatsu Toru,
Yoshikawa Hideki,
Itoh Kazuyuki
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.11.014
Subject(s) - cdc42 , rac1 , microbiology and biotechnology , mesenchymal stem cell , chemistry , cell migration , cellular differentiation , runx2 , cell , rho associated protein kinase , downregulation and upregulation , gtpase , phosphorylation , osteoblast , signal transduction , biology , biochemistry , in vitro , gene
We showed that the migration, morphology and adhesiveness of undifferentiated mesenchymal cells dramatically changed during osteogenic differentiation. The migration of these cells was transiently upregulated early in osteogenic differentiation. At a later stage, migration was decreased but adhesiveness was increased. Furthermore, Cdc42 and Rac1 Rho‐family small GTPases were activated at early stages of differentiation and the phosphorylation level of FAK decreased as differentiation progressed. We also showed cell migration was promoted by inhibition of the Rho‐ROCK‐myosin signaling. Finally, using a mouse model of ectopic bone formation, we confirmed that treatment with ROCK inhibitor, Y‐27632 increased cell movement into bone formation sites, resulting in enhanced osteogenesis. These results provide a new insight into the link between cell migration and osteogenic differentiation.