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Evaluation and comparison of protein splicing by exogenous inteins with foreign exteins in Escherichia coli
Author(s) -
Ellilä Simo,
Jurvansuu Jaana Maria,
Iwaï Hideo
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.10.005
Subject(s) - intein , protein splicing , rna splicing , pyrococcus horikoshii , protein engineering , computational biology , trans splicing , escherichia coli , biology , genetics , gene , biochemistry , rna , enzyme
Protein splicing catalyzed by inteins has enabled various biotechnological applications such as protein ligation. Successful applications of inteins are often limited by splicing efficiency. Here, we report the comparison of protein splicing between 20 different inteins from various organisms in identical contexts to identify robust inteins with foreign exteins. We found that RadA intein from Pyrococcus horikoshii and an engineered DnaB intein from Nostoc punctiforme demonstrated an equally efficient splicing activity to the previously reported highly efficient DnaE intein from Nostoc punctiforme . The newly identified inteins with efficient cis ‐splicing activity can be good starting points for the further development of new protein engineering tools.