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Two non‐vesicular ATP release pathways in the mouse erythrocyte membrane
Author(s) -
Qiu Feng,
Wang Junjie,
Spray David C.,
Scemes Eliana,
Dahl Gerhard
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.09.033
Subject(s) - pannexin , microbiology and biotechnology , vesicle , chemistry , adenosine triphosphate , biophysics , exocytosis , biochemistry , membrane , biology , intracellular , gap junction , connexin
Erythrocytes are exceptionally suited for analysis of non‐exocytotic release mechanisms of ATP, because these cells under physiological conditions lack vesicles. Previous studies have indicated, that Pannexin1 (Panx1) provides a key ATP permeation pathway in many cell types, including human and frog erythrocytes. Here we show that erythrocytes of Panx1 −/− mice lend further support to this conclusion. However, ATP release, although attenuated, was still observed in Panx1 −/− mouse erythrocytes. In contrast to Panx1 +/+ cells, this release was not correlated with uptake of extracellularly applied dyes, was insensitive to Panx1 channel blockers, and was inhibited by dipyridamole and stimulated by iloprost. Thus, in erythrocytes, two independent pathways mediate the release of ATP. We also show that glyburide is a strong inhibitor of Panx1 channels.