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MiR‐143 enhances adipogenic differentiation of 3T3‐L1 cells through targeting the coding region of mouse pleiotrophin
Author(s) -
Yi Can,
Xie Wei-dong,
Li Fu,
Lv Qing,
He Jie,
Wu Jiangbin,
Gu Dayong,
Xu Naihan,
Zhang Yaou
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.09.015
Subject(s) - pleiotrophin , adipogenesis , microbiology and biotechnology , gene knockdown , microrna , chemistry , biology , gene , growth factor , genetics , mesenchymal stem cell , receptor
Adipogenic differentiation of preadipocytes is a complex process regulated by various factors including miRNAs and cytokines. MiR‐143 is a well known miRNA that enhances adipogenesis. Pleiotrophin (PTN), a heparin‐binding growth factor, plays a negative role in adipogenesis. In this investigation, we demonstrate that PTN is a target gene of miR‐143 during adipogenic differentiation in 3T3‐L1 preadipocytes. MiR‐143 down regulates PTN expression through interaction with a target site of miR‐143 in the coding region of mouse PTN. The rare codons upstream of the target site regulate miR143‐induced translational knockdown of PTN, which provides more insight into the mechanism of adipogenic differentiation.