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Novel effects of CCN3 that may direct the differentiation of chondrocytes
Author(s) -
Janune Danilo,
Kubota Satoshi,
Nishida Takashi,
Kawaki Harumi,
Perbal Bernard,
Iida Seiji,
Takigawa Masaharu
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.08.024
Subject(s) - chondrogenesis , chondrocyte , endochondral ossification , cartilage , microbiology and biotechnology , chemistry , phenotype , in vitro , sox9 , ossification , matrix (chemical analysis) , medicine , endocrinology , anatomy , biology , biochemistry , gene , transcription factor , chromatography
Identification and characterization of local molecules directing the differentiation of chondrocytes to either transient or permanent cartilage are major issues in cartilage biology. Here, we found CCN family protein 3 (CCN3) was abundantly produced in rat developing epiphyseal cartilage. Evaluations in vitro showed that CCN3 repressed epiphyseal chondrocyte proliferation, while promoting matrix production in multiple assays performed. Furthermore, CCN3 enhanced the articular chondrocytic phenotype; whereas it repressed the one representing endochondral ossification. Additionally, the phenotype of growth plate chondrocytes and chondrogenic progenitors also appeared to be affected by CCN3 in a similar manner. These findings suggest a significant role of CCN3 in inducing chondrocytes to articular ones during joint formation.