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Free sphingobases induce RBOHD‐dependent reactive oxygen species production in Arabidopsis leaves
Author(s) -
Peer Markus,
Bach Matthias,
Mueller Martin J.,
Waller Frank
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.08.016
Subject(s) - reactive oxygen species , arabidopsis , nadph oxidase , programmed cell death , pseudomonas syringae , microbiology and biotechnology , respiratory burst , biology , biochemistry , chemistry , mutant , apoptosis , gene
Sphingolipids are implied in several regulatory processes, including cell death. Levels of the free sphingobase t18:0 (phytosphingosine) increase in Arabidopsis in response to the bacterial pathogen Pseudomonas syringae . To gain information on sphingobase‐induced signaling, we determined kinetics of leaf reactive oxygen species (ROS) levels and cell death in response to specific sphingobases. t18:0, d18:0 and d17:1, but not d20:0, induced ROS and cell death within 1.5–2 h. Early sphingobase‐induced ROS production was independent of cell death induction and required the NADPH oxidase Respiratory Burst Oxidase Homolog D (RBOHD). Specific sphingobases can therefore induce cell death and require RBOHD for early ROS induction in plants.