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MiR‐140 is co‐expressed with Wwp2‐C transcript and activated by Sox9 to target Sp1 in maintaining the chondrocyte proliferation
Author(s) -
Yang Jun,
Qin Shengying,
Yi Chengqing,
Ma Gang,
Zhu Huang,
Zhou Wenrong,
Xiong Yuyu,
Zhu Xuming,
Wang Yujiong,
He Lin,
Guo Xizhi
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.08.013
Subject(s) - chondrogenesis , chondrocyte , gene knockdown , microbiology and biotechnology , sox9 , biology , microrna , primary transcript , wnt signaling pathway , gene isoform , regulator , intron , activator (genetics) , repressor , cartilage , gene , gene expression , signal transduction , genetics , anatomy , mesenchymal stem cell , alternative splicing
MiR‐140 is a microRNA specially involved in chondrogenesis and osteoarthritis pathogenesis. However, its transcriptional regulation and target genes in cartilage development are not fully understood. Here we detected that miR‐140 was uniquely expressed in chondrocyte and suppressed by Wnt/β‐catenin signalling. The miR‐140 primary transcript was an intron‐retained RNA co‐expressed with Wwp2‐C isoform, which was directly induced by Sox9 through binding to the intron 10 of Wwp2 gene. Knockdown of miR‐140 in limb bud micromass cultures resulted in arrest of chondrogenic proliferation. Sp1, the activator of the cell cycle regulator p15 INK4b , was identified as a target of miR‐140 in maintaining the chondrocyte proliferation. Collectively, our findings expand our understanding of the transcriptional regulation and the chondrogenic role of miR‐140 in chondrogenesis.
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