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Ago1 and Ago2 differentially affect cell proliferation, motility and apoptosis when overexpressed in SH‐SY5Y neuroblastoma cells
Author(s) -
Parisi Chiara,
Giorgi Corinna,
Batassa Enrico Maria,
Braccini Laura,
Maresca Giovanna,
D'agnano Igea,
Caputo Viviana,
Salvatore Annamaria,
Pietrolati Flavia,
Cogoni Carlo,
Catalanotto Caterina
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.08.003
Subject(s) - motility , argonaute , microbiology and biotechnology , biology , cell growth , cell cycle , apoptosis , microrna , function (biology) , cell , neuroblastoma , cancer research , cell culture , gene , rna interference , rna , genetics
Argonaute are a conserved class of proteins central to the microRNA pathway. We have highlighted a novel and non‐redundant function of Ago1 versus Ago2; the two core factors of the miRNA‐associated RISC complex. Stable overexpression of Ago1 in neuroblastoma cells causes the cell cycle to slow down, a decrease in cellular motility and a stronger apoptotic response upon UV irradiation. These effects, together with a significant increase in p53 levels, suggest that Ago1 may act as a tumor‐suppressor factor, a function also supported by GEO Profiles microarrays that inversely correlate Ago1 expression levels with cell proliferation rates.