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Functional analysis of metal tolerance proteins isolated from Zn/Cd hyperaccumulating ecotype and non‐hyperaccumulating ecotype of Sedum alfredii Hance
Author(s) -
Zhang Min,
Senoura Takeshi,
Yang Xiaoe,
Nishizawa Naoko K.
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.07.013
Subject(s) - ecotype , shoot , hyperaccumulator , crassulaceae , botany , sedum , biology , gene , heterologous expression , biochemistry , recombinant dna , phytoremediation , ecology , contamination
The Zn/Cd hyperaccumulating ecotype (HE) of Sedum alfredii Hance can accumulate 24‐ and 28‐fold higher leaf and stem Zn concentrations when compared with the non‐hyperaccumulating ecotype (NHE) of Sedum . Heterologous expression of a metal tolerance protein (MTP1) encoding gene from HE plants ( SaMTP1 ) or the homologous gene from NHE plants ( SnMTP1 ) suppressed Zn 2+ hypersensitivity in the Δzrc1 yeast mutant. In plants, SaMTP1 localized to the tonoplast. Furthermore, MTP1 transcript level in the shoot of HE plants was more than 80‐fold higher than that of NHE plants. The transcript level of SaMTP1 in shoot was up‐regulated 1‐fold by Zn 2+ while the expression of SnMTP1 was slightly inhibited. These data suggest that SaMTP1 can play an important role in Zn accumulation in HE plants.