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RuvBl2 cooperates with Ets2 to transcriptionally regulate hTERT in colon cancer
Author(s) -
Flavin Padraic,
Redmond Aisling,
McBryan Jean,
Cocchiglia Sinead,
Tibbitts Paul,
Fahy-Browne Patrick,
Kay Elaine,
Treumann Achim,
Perrem Kilian,
McIlroy Marie,
Hill Arnold D.K.,
Young Leonie S.
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.07.005
Subject(s) - telomerase reverse transcriptase , telomerase , telomere , cancer research , biology , transcriptional regulation , colorectal cancer , cancer , transcription factor , gene , genetics
Human cancers utilise telomerase to maintain telomeres and prohibit cell senescence. Human telomerase reverse transcriptase (hTERT), an essential component of this complex, is regulated at the level of gene transcription. Using SILAC‐proteomic analysis and molecular studies, we identified the AAA+ ATPase, RuvBl2 as a transcriptional regulator of hTERT and established that this regulation is through cooperation with Ets‐2. In colon cancer patients, nuclear expression of RuvBl2 associated with nuclear expression of hTERT, pEts2 and advanced nodal disease ( P < 0.01, P = 0.05 and P = 0.03 respectively, n = 170). These data firmly implicate RuvBl2 in Ets2 mediated regulation of hTERT in colon cancer which has functional and clinical consequences.