Premium
RNA interference‐produced autoregulation of inducible nitric oxide synthase expression
Author(s) -
Feng Chenzhuo,
Cao Lin,
Zuo Zhiyi
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.06.032
Subject(s) - small hairpin rna , rna interference , transfection , microbiology and biotechnology , nitric oxide synthase , gene expression , lipopolysaccharide , nitric oxide , gene silencing , rna , chemistry , biology , plasmid , gene , small interfering rna , biochemistry , immunology , endocrinology
Vector‐mediated delivery of short‐hairpin RNA (shRNA) to regulate gene expression holds a great therapeutic promise. We hypothesize that gene expression can be autoregulated with RNA interference. We used inducible nitric oxide synthase (iNOS) as a gene model to test this hypothesis. Lipopolysaccharide dose‐dependently increased iNOS in rat aortic smooth muscle cells and the nitrite production from these cells. These increases were attenuated in cells transfected with plasmids containing code for iNOS shRNA whose expression was controlled by an iNOS promoter. The production of shRNA was lipopolysaccharide dose‐dependent. The lipopolysaccharide‐induced iNOS expression in rat C6 glioma cells also was attenuated by transfection with plasmids containing the iNOS shRNA code. These results provide proof‐of‐concept evidence for using RNA interference technique to achieve autoregulation of gene expression.