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Epigenetic regulation of microRNA‐375 and its role in melanoma development in humans
Author(s) -
Mazar Joseph,
DeBlasio Dan,
Govindarajan Subramaniam S.,
Zhang Shaojie,
Perera Ranjan J.
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.06.025
Subject(s) - melanoma , microrna , epigenetics , ectopic expression , cancer research , dna methylation , cpg site , biology , motility , cell growth , methylation , cell culture , microbiology and biotechnology , gene expression , gene , genetics
To identify epigenetically regulated miRNAs in melanoma, we treated a stage 3 melanoma cell line WM1552C, with 5AzadC and/or 4‐PBA. Several hypermethylated miRNAs were detected, one of which, miR‐375, was highly methylated and was studied further. Minimal CpG island methylation was observed in melanocytes, keratinocytes, normal skin, and nevus but hypermethylation was observed in patient tissue samples from primary, regional, distant, and nodular metastatic melanoma. Ectopic expression of miR‐375 inhibited melanoma cell proliferation, invasion, and cell motility, and induced cell shape changes, strongly suggesting that miR‐375 may have an important function in the development and progression of human melanomas.