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Evidence for direct binding of glycerol to photosystem I
Author(s) -
Hussels Martin,
Brecht Marc
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.06.018
Subject(s) - glycerol , buffer (optical fiber) , photosystem ii , chemistry , buffer solution , fluorescence , fluorescence spectroscopy , analytical chemistry (journal) , chromatography , photosynthesis , biochemistry , telecommunications , physics , quantum mechanics , computer science
The interaction between glycerol and photosystem I (PSI) was investigated using low temperature single‐molecule spectroscopy. PSI complexes were dissolved in three different solutions: in buffer solution, in 66% glycerol/buffer solution, and in 66% glycerol/buffer solution that was afterwards diluted by buffer; the final glycerol concentration was <1‰. Mean fluorescence spectra and intercomplex heterogeneity of PSI complexes in 66% glycerol/buffer solution and in the re‐diluted solution show high similarity, but differ from complexes in buffer solution indicating that the glycerol concentration is not the determining factor modifying the spectral properties. However, the exposure of PSI to a high glycerol concentration during sample preparation affects PSI and the effect is maintained if glycerol is removed from the solution.