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The PPR‐DYW proteins are required for RNA editing of rps14 , cox1 and nad5 transcripts in Physcomitrella patens mitochondria
Author(s) -
Uchida Masato,
Ohtani Shotaro,
Ichinose Mizuho,
Sugita Chieko,
Sugita Mamoru
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.06.009
Subject(s) - pentatricopeptide repeat , physcomitrella patens , rna editing , biology , rna , gene , rna binding protein , genetics , microbiology and biotechnology , mutant
We identified two DYW subclass pentatricopeptide repeat (PPR) proteins, PpPPR_78 and PpPPR_79, as RNA editing factors in the moss Physcomitrella patens . Disruption of each gene by homologous recombination revealed that PpPPR_78 was involved in RNA editing at the rps14 ( rps14 ‐C137) and cox1 ( cox1 ‐C755) sites and PpPPR_79 at the nad5‐1 ( nad5 ‐C598) site in the mitochondrial transcripts. RNA editing defects did not affect transcript patterns of the target genes. Thus, DYW subclass PPR proteins seem to be site‐specific trans ‐acting factors for RNA editing.