Premium
Ubiquitin‐family modifications in the replication of DNA damage
Author(s) -
Lehmann Alan R.
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.06.005
Subject(s) - proliferating cell nuclear antigen , ubiquitin , dna polymerase , polymerase , dna replication , biology , microbiology and biotechnology , dna repair , chromatin , genetics , dna , gene
The cell uses specialised Y‐family DNA polymerases or damage avoidance mechanisms to replicate past damaged sites in DNA. These processes are under complex regulatory systems, which employ different types of post‐translational modification. All the Y‐family polymerases have ubiquitin binding domains that bind to mono‐ubiquitinated PCNA to effect the switching from replicative to Y‐family polymerase. Ubiquitination and de‐ubiquitination of PCNA are tightly regulated. There is also evidence for another as yet unidentified ubiquitinated protein being involved in recruitment of Y‐family polymerases to chromatin. Poly‐ubiquitination of PCNA stimulates damage avoidance, and, at least in yeast, PCNA is SUMOylated to prevent unwanted recombination events at the replication fork. The Y‐family polymerases themselves can be ubiquitinated and, in the case of DNA polymerase η, this results in the polymerase being excluded from chromatin.