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NAADP mediates ATP‐induced Ca 2+ signals in astrocytes
Author(s) -
Barceló-Torns Miquel,
Lewis Alexander M.,
Gubern Albert,
Barneda David,
Bloor-Young Duncan,
Picatoste Fernado,
Churchill Grant C.,
Claro Enrique,
Masgrau Roser
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.05.062
Subject(s) - second messenger system , intracellular , chemistry , biochemistry , microbiology and biotechnology , lysosome , receptor , neurotransmission , biophysics , biology , enzyme
Intracellular Ca 2+ signals provide astrocytes with a specific form of excitability that enables them to regulate synaptic transmission. In this study, we demonstrate that NAADP‐AM, a membrane‐permeant analogue of the new second messenger nicotinic acid‐adenine dinucleotide phosphate (NAADP), mobilizes Ca 2+ in astrocytes and that the response is blocked by Ned‐19, an antagonist of NAADP signalling. We also show that NAADP receptors are expressed in lysosome‐related acidic vesicles. Pharmacological disruption of either NAADP or lysosomal signalling reduced Ca 2+ responses induced by ATP and endothelin‐1, but not by bradykinin. Furthermore, ATP increased endogenous NAADP levels. Overall, our data provide evidence for NAADP being an intracellular messenger for agonist‐mediated calcium signalling in astrocytes.