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c‐Jun/AP‐1 transcription factor regulates laminin‐1‐induced neurite outgrowth in human bone marrow mesenchymal stem cells: Role of multiple signaling pathways
Author(s) -
Mruthyunjaya S.,
Rumma Manchanda,
Ravibhushan Godbole,
Anjali Shiras,
Padma Shastry
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.04.072
Subject(s) - neurite , microbiology and biotechnology , mesenchymal stem cell , chemistry , pi3k/akt/mtor pathway , mapk/erk pathway , signal transduction , transcription factor , c jun , biology , gene , in vitro , biochemistry
In this study, we demonstrated that laminin‐1 (LM‐1) induces neurite outgrowth and enhances the expression of neurofilament‐L and MAP2 in human bone marrow mesenchymal stem cells (MSCs). The c‐Jun transcription factor was strongly activated by LM‐1 during neurite induction. Suppression of c‐Jun inhibited the expression of the c‐Jun target genes α6 integrin and neurofilament‐L, resulting in the loss of neurite outgrowth. Additionally, we found that the LM‐1‐α6 integrin interaction stimulated phosphorylation of FAK, leading to the activation of JNK and Akt. Pharmacological inhibition of these pathways blocked c‐Jun activation and neurite outgrowth. Collectively, our findings suggest that c‐Jun/AP‐1 activity mediated by JNK, PI3K/Akt and ERK pathways is required for LM‐1‐induced neurite outgrowth in human bone marrow MSCs.