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MicroRNA‐142‐3p, a new regulator of RAC1, suppresses the migration and invasion of hepatocellular carcinoma cells
Author(s) -
Wu Lifen,
Cai Chunli,
Wang Xinghua,
Liu Min,
Li Xin,
Tang Hua
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.03.067
Subject(s) - rac1 , microrna , regulator , carcinogenesis , cancer research , biology , hepatocellular carcinoma , messenger rna , untranslated region , microbiology and biotechnology , gene , signal transduction , genetics
RAC1 regulates a diverse array of cellular events including migration and invasion. MicroRNAs (miRNAs) have a key role in the regulation of gene expression. In this study, we demonstrated that microRNA‐142‐3p (miR‐142‐3p) acted as a negative regulator of human RAC1. Overexpression of miR‐142‐3p decreased RAC1 mRNA and protein levels. Moreover, the overexpression of miR‐142‐3p suppressed, while blocking of miR‐142‐3p increased colony formation, migration and invasion in hepatocellular carcinoma (HCC) cell lines (QGY‐7703 and SMMC‐7721). RAC1 overexpression without the 3′untranslated region abolished the effect of miR‐142‐3p in the QGY‐7703 and SMMC‐7721 cells. These results demonstrated that miR‐142‐3p directly and negatively regulates RAC1 in HCC cells, which highlights the importance of miRNAs in tumorigenesis.