Premium
Cyanidioschyzon merolae ferredoxin: A high resolution crystal structure analysis and its thermal stability
Author(s) -
Yamaoka Asami,
Ozawa Yukiko,
Ueno Yuko,
Endo Tsukasa,
Morimoto Yukio,
Urushiyama Akio,
Ohmori Daijiro,
Imai Takeo
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.03.020
Subject(s) - spinacia , ferredoxin , thermophile , spinach , crystallography , covalent bond , chemistry , crystal structure , biochemistry , chloroplast , enzyme , organic chemistry , gene
Cyanidioschyzon merolae (Cm) is a single‐cell red alga that grows under moderately thermophilic (40–50 °C), acidic (pH 1–3) conditions. We purified a Cm ferredoxin (Fd) that was characterized as a plant‐type [2Fe–2S] Fd by physicochemical techniques. X‐ray crystallography revealed that the overall three‐dimensional structure of CmFd was highly similar to, but slightly different from, the [2Fe–2S] Fd from Spinacia oleracea , whose growth temperature is 15–20 °C. Therefore, slight structural differences, including non‐covalent‐bond number and amino acid sequence, may underlie the differential thermostabilities of the plant‐type Fds.