PU.1 is a major transcriptional activator of the tumour suppressor gene  LIMD1 | Zendy

Foxler Daniel E. | Zendy; James Victoria | Zendy; Shelton Samuel J. | Zendy; de A. Vallim Thomas Q. | Zendy; Shaw Peter E. | Zendy; Sharp Tyson V. | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

PU.1 is a major transcriptional activator of the tumour suppressor gene LIMD1

Author(s) -

Foxler Daniel E.,

James Victoria,

Shelton Samuel J.,

de A. Vallim Thomas Q.,

Shaw Peter E.,

Sharp Tyson V.

Publication year - 2011

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/j.febslet.2011.03.013

Subject(s) - gene silencing , transcription factor , activator (genetics) , gene , transcription (linguistics) , promoter , microrna , biology , gene expression , cancer research , genetics , philosophy , linguistics

LIMD1 is a tumour suppressor gene (TSG) down regulated in ∼80% of lung cancers with loss also demonstrated in breast and head and neck squamous cell carcinomas. LIMD1 is also a candidate TSG in childhood acute lymphoblastic leukaemia. Mechanistically, LIMD1 interacts with pRB, repressing E2F‐driven transcription as well as being a critical component of microRNA‐mediated gene silencing. In this study we show a CpG island within the LIMD1 promoter contains a conserved binding motif for the transcription factor PU.1. Mutation of the PU.1 consensus reduced promoter driven transcription by 90%. ChIP and EMSA analysis demonstrated that PU.1 specifically binds to the LIMD1 promoter. siRNA depletion of PU.1 significantly reduced endogenous LIMD1 expression, demonstrating that PU.1 is a major transcriptional activator of LIMD1.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research