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Production of superoxide in chloroplast thylakoid membranes
Author(s) -
Kozuleva Marina,
Klenina Irina,
Proskuryakov Ivan,
Kirilyuk Igor,
Ivanov Boris
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.03.004
Subject(s) - thylakoid , superoxide , chemistry , radical , electron transport chain , chloroplast , biophysics , nitroxide mediated radical polymerization , photochemistry , superoxide dismutase , membrane , biochemistry , biology , oxidative stress , enzyme , organic chemistry , radical polymerization , copolymer , gene , polymer
Accumulation of nitroxide radicals, DCP or TMT , under illumination of a thylakoid suspension containing either hydrophilic, DCP‐H, or lipophilic, TMT‐H, cyclic hydroxylamines that have high rate constants of the reaction with superoxide radicals, was measured using ESR. A slower accumulation of TMT in contrast with DCP accumulation was explained by re‐reduction of TMT by the carriers of the photosynthetic electron transport chain within the membrane. Superoxide dismutase suppressed TMT accumulation to a lesser extent than DCP accumulation. The data are interpreted as evidencing the production of intramembrane superoxide in thylakoids.