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Label‐free optical biosensor with microfluidics for sensing ligand‐directed functional selectivity on trafficking of thrombin receptor
Author(s) -
Goral Vasiliy,
Wu Qi,
Sun Haiyan,
Fang Ye
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.03.003
Subject(s) - biosensor , microfluidics , functional selectivity , receptor , ligand (biochemistry) , agonist , chemistry , thrombin , drug discovery , g protein coupled receptor , small molecule , biophysics , microbiology and biotechnology , nanotechnology , biochemistry , biology , materials science , immunology , platelet
Ligand‐directed functional selectivity has stimulated much interest in the molecular delineation of drug pharmacology and the process of drug discovery. Here we describe the development of a label‐free optical biosensor with microfluidics for whole cell sensing. The microfluidics controls the duration of agonist exposure and of the functional recovery of activated protease activated receptor‐1 (PAR 1 ). The biosensor manifests that the persistency of PAR 1 signaling is dependent on the agonists, which, in turn, affects the receptor resensitization process. This study demonstrates a new means to differentiate ligand‐directed functional selectivity on trafficking of receptors.